Two spectrally different forms of the phytochrome chromophore extracted from etiolated oat seedlings
نویسندگان
چکیده
منابع مشابه
Phytochrome control of two low-irradiance responses in etiolated oat seedlings.
Light-induced coleoptile stimulation and mesocotyl suppression in etiolated Avena sativa (cv. Lodi) has been quantitated. Etiolated seedlings showed the greatest response to light when they were illuminated 48 to 56 hours after imbibition. Two low-irradiance photoresponses for each tissue have been described. Red light was 10 times more effective than green and 1,000 times more effective than f...
متن کاملDistribution of Phytochrome in Etiolated Seedlings.
'I'lle l)lalt p)ignent, phytochrome regulates a large numl)er of l)hysiological processes in higher plaints (14). T'he pigment is nmost readily detected in darkgrown see(llings both because it is normally present in higher conlcenitrationis than in light-grown seedlinigs (8, 11), and because dark-grown seedlinigs contain only smlall amounts of other pigments absorbing ligfit ini the same spectr...
متن کاملEvidence for bound phytochrome in oat seedlings.
Phytochrome is consistently observed in pellets centrifuged from homogenates of etiolated, 5-day-old oat seedlings. The majority of pigment associated with the pellet cannot be removed by buffer washes, nor can appreciable quantities of additional phytochrome be adsorbed onto the sedimented material. Over 70% of phytochrome in the pellet is released by 1% Triton X-100.Storage at 0 degrees , irr...
متن کاملThe aggregation States of phytochrome from etiolated rye and oat seedings.
Initial extracts from etiolated plants contained two aggregates of phytochrome. A major fraction was almost excluded by Sephadex G-200 and was within the fractionation range of Sepharose 4 B. A minor fraction was within the fractionation range of Sephadex G-200. Modifications of a previous isolation procedure which allowed retention of this aggregation state are reported. With respect to gel fi...
متن کاملOat Phytochrome Is Biologically Active in Transgenic Tomatoes.
To determine the functional homology between phytochromes from evolutionarily divergent species, we used the cauliflower mosaic virus 35S promoter to express a monocot (oat) phytochrome cDNA in a dicot plant (tomato). Immunoblot analysis shows that more than 50% of the transgenic tomato plants synthesize the full-length oat phytochrome polypeptide. Moreover, leaves of light-grown transgenic pla...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1070603